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1x beta mercaptoethanol  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 1x beta mercaptoethanol
    1x Beta Mercaptoethanol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2+beta+mercaptoethanol/2-Mercaptoethanol%2C+98%2B%25/10__3390_slash_cancers18040599-47-41-47
    Average 99 stars, based on 1 article reviews
    1x beta mercaptoethanol - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Irradiation:

    Article Title: TIM-3 blockade enhances ex vivo stimulated allogeneic NK cell therapy for relapsed murine neuroblastoma after hematopoietic cell transplant
    Article Snippet: NK cells were isolated using the NK cell isolation kit (Miltenyi Biotec, Auburn, CA) by negative selection on the AutoMACs Pro (Miltenyi Biotec). .. NK cells were expanded and activated in the presence or absence of irradiated (100Gy) AgN2a-4P at a 1:1 ratio and cultured in RPMI medium (Corning Life Sciences, Durham, NC) containing 10 ng/ml IL-15/IL-15Rα conjugate, comprised of recombinant mouse IL-15 protein (R&D Systems, Minneapolis, MN) and recombinant mouse IL-15Ralpha Fc chimera protein (R&D Systems), 10% fetal bovine serum (GeminiBio, Sacramento, CA) with 50 IU/mL penicillin/streptomycin (Lonza), 0.11 mM 2-beta-mercaptoethanol (Gibco, Carlsbad, CA), 1X MEM nonessential amino acids (Corning), 25 mM HEPES buffer (Corning), and 1 mM sodium pyruvate (Corning). ..

    Article Title: TIM-3 blockade enhances ex vivo stimulated allogeneic NK cell therapy for relapsed murine neuroblastoma after hematopoietic cell transplant
    Article Snippet: NK cells were isolated using the NK cell isolation kit (Miltenyi Biotec, Auburn, California, USA) by negative selection on the AutoMACs Pro (Miltenyi Biotec). .. NK cells were expanded and activated in the presence or absence of irradiated (100 Gy) AgN2a-4P at a 1:1 ratio and cultured in Roswell Park Memorial Institute (RPMI) medium (Corning Life Sciences, Durham, North Carolina, USA) containing 10 ng/mL IL-15/IL-15 receptor alpha (IL-15Rα) conjugate, comprised of recombinant mouse IL-15 protein (R&D Systems, Minneapolis, Minnesota, USA) and recombinant mouse IL-15Rα Fc chimera protein (R&D Systems), 10% fetal bovine serum (GeminiBio, Sacramento, California, USA) with 50 IU/mL penicillin/streptomycin (Lonza), 0.11 mM 2-beta-mercaptoethanol (Gibco, Carlsbad, California, USA), 1× MEM non-essential amino acids (Corning), 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (Corning), and 1 mM sodium pyruvate (Corning). ..

    Cell Culture:

    Article Title: TIM-3 blockade enhances ex vivo stimulated allogeneic NK cell therapy for relapsed murine neuroblastoma after hematopoietic cell transplant
    Article Snippet: NK cells were isolated using the NK cell isolation kit (Miltenyi Biotec, Auburn, CA) by negative selection on the AutoMACs Pro (Miltenyi Biotec). .. NK cells were expanded and activated in the presence or absence of irradiated (100Gy) AgN2a-4P at a 1:1 ratio and cultured in RPMI medium (Corning Life Sciences, Durham, NC) containing 10 ng/ml IL-15/IL-15Rα conjugate, comprised of recombinant mouse IL-15 protein (R&D Systems, Minneapolis, MN) and recombinant mouse IL-15Ralpha Fc chimera protein (R&D Systems), 10% fetal bovine serum (GeminiBio, Sacramento, CA) with 50 IU/mL penicillin/streptomycin (Lonza), 0.11 mM 2-beta-mercaptoethanol (Gibco, Carlsbad, CA), 1X MEM nonessential amino acids (Corning), 25 mM HEPES buffer (Corning), and 1 mM sodium pyruvate (Corning). ..

    Article Title: TIM-3 blockade enhances ex vivo stimulated allogeneic NK cell therapy for relapsed murine neuroblastoma after hematopoietic cell transplant
    Article Snippet: NK cells were isolated using the NK cell isolation kit (Miltenyi Biotec, Auburn, California, USA) by negative selection on the AutoMACs Pro (Miltenyi Biotec). .. NK cells were expanded and activated in the presence or absence of irradiated (100 Gy) AgN2a-4P at a 1:1 ratio and cultured in Roswell Park Memorial Institute (RPMI) medium (Corning Life Sciences, Durham, North Carolina, USA) containing 10 ng/mL IL-15/IL-15 receptor alpha (IL-15Rα) conjugate, comprised of recombinant mouse IL-15 protein (R&D Systems, Minneapolis, Minnesota, USA) and recombinant mouse IL-15Rα Fc chimera protein (R&D Systems), 10% fetal bovine serum (GeminiBio, Sacramento, California, USA) with 50 IU/mL penicillin/streptomycin (Lonza), 0.11 mM 2-beta-mercaptoethanol (Gibco, Carlsbad, California, USA), 1× MEM non-essential amino acids (Corning), 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (Corning), and 1 mM sodium pyruvate (Corning). ..

    Article Title: L-Type Ca(2+) Channels and SK Channels in Mouse Embryonic Stem Cells and Their Contribution to Cell Proliferation.
    Article Snippet: Cell Culture The mESC line D3 was purchased from American Type Culture Collection (ATCC, USA). .. Cells were cultured on gelatine-coated flasks with high-glucose Dulbecco’s mod- ified Eagle’s medium (DMEM, Gibco/BRL, Grand Island, NY, http://www.invitrogen.com) supplemented with 15 % fetal bovine serum FBS (Gibco), 1 % non-essential amino acids (Gibco/BRL), 0.1 mM 2-beta-mercaptoethanol (Gibco/BRL), 1 mM sodium pyruvate, 100 U/ml penicillin, and 0.1 mg/ml streptomycin, and maintained at 37 8C in an atmosphere of 95 % air, 5 % CO2. .. The undifferentiated state was maintained by 1,000 U/ml recombinant LIF (Gibco).

    Article Title: Variability in the virulence of specific Mycobacterium tuberculosis clinical isolates alters the capacity of human dendritic cells to signal for T cells
    Article Snippet: .. In order to generate MDDCs, mononuclear cells (5 × 10 6 ) were seeded in a 75 cm 2 flask using RPMI-1640 medium supplemented with 10% foetal calf serum (FCS) (Gibco Life Technologies, USA), 1% pyruvate (Sigma Aldrich, USA), 0.1% 2-beta-mercaptoethanol (Gibco Life Technologies, USA), recombinant IL-4 (15 ng/mL) (Peprotech Inc., USA), and granulocyte macrophage colony stimulating factor (GM-CSF, 200 ng/mL) (GRAMAL, Probiomed Lab, Mexico), and cultured at 37oC in a humidified atmosphere at 5% CO 2 . ..

    Recombinant:

    Article Title: TIM-3 blockade enhances ex vivo stimulated allogeneic NK cell therapy for relapsed murine neuroblastoma after hematopoietic cell transplant
    Article Snippet: NK cells were isolated using the NK cell isolation kit (Miltenyi Biotec, Auburn, CA) by negative selection on the AutoMACs Pro (Miltenyi Biotec). .. NK cells were expanded and activated in the presence or absence of irradiated (100Gy) AgN2a-4P at a 1:1 ratio and cultured in RPMI medium (Corning Life Sciences, Durham, NC) containing 10 ng/ml IL-15/IL-15Rα conjugate, comprised of recombinant mouse IL-15 protein (R&D Systems, Minneapolis, MN) and recombinant mouse IL-15Ralpha Fc chimera protein (R&D Systems), 10% fetal bovine serum (GeminiBio, Sacramento, CA) with 50 IU/mL penicillin/streptomycin (Lonza), 0.11 mM 2-beta-mercaptoethanol (Gibco, Carlsbad, CA), 1X MEM nonessential amino acids (Corning), 25 mM HEPES buffer (Corning), and 1 mM sodium pyruvate (Corning). ..

    Article Title: TIM-3 blockade enhances ex vivo stimulated allogeneic NK cell therapy for relapsed murine neuroblastoma after hematopoietic cell transplant
    Article Snippet: NK cells were isolated using the NK cell isolation kit (Miltenyi Biotec, Auburn, California, USA) by negative selection on the AutoMACs Pro (Miltenyi Biotec). .. NK cells were expanded and activated in the presence or absence of irradiated (100 Gy) AgN2a-4P at a 1:1 ratio and cultured in Roswell Park Memorial Institute (RPMI) medium (Corning Life Sciences, Durham, North Carolina, USA) containing 10 ng/mL IL-15/IL-15 receptor alpha (IL-15Rα) conjugate, comprised of recombinant mouse IL-15 protein (R&D Systems, Minneapolis, Minnesota, USA) and recombinant mouse IL-15Rα Fc chimera protein (R&D Systems), 10% fetal bovine serum (GeminiBio, Sacramento, California, USA) with 50 IU/mL penicillin/streptomycin (Lonza), 0.11 mM 2-beta-mercaptoethanol (Gibco, Carlsbad, California, USA), 1× MEM non-essential amino acids (Corning), 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (Corning), and 1 mM sodium pyruvate (Corning). ..

    Article Title: Variability in the virulence of specific Mycobacterium tuberculosis clinical isolates alters the capacity of human dendritic cells to signal for T cells
    Article Snippet: .. In order to generate MDDCs, mononuclear cells (5 × 10 6 ) were seeded in a 75 cm 2 flask using RPMI-1640 medium supplemented with 10% foetal calf serum (FCS) (Gibco Life Technologies, USA), 1% pyruvate (Sigma Aldrich, USA), 0.1% 2-beta-mercaptoethanol (Gibco Life Technologies, USA), recombinant IL-4 (15 ng/mL) (Peprotech Inc., USA), and granulocyte macrophage colony stimulating factor (GM-CSF, 200 ng/mL) (GRAMAL, Probiomed Lab, Mexico), and cultured at 37oC in a humidified atmosphere at 5% CO 2 . ..

    other:

    Article Title: A method of processing nasopharyngeal swabs to enable multiple testing
    Article Snippet: ( ) consisted of cutting off the distal swab tip (~0.5 cm) with clean scissors immediately after collection, placing the tip into a cryovial with 350μL of RLT Plus® (Qiagen) with 350 μL 2-beta mercaptoethanol (Invitrogen), and vortexing the cryovial for 30 seconds, and transporting the sample to the laboratory on ice for storage at −80° C. The proximal part of the swab was placed in ESwabTM liquid Amies and processed as previously described.




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